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anti ulk1 d8h5  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti ulk1 d8h5
    Anti Ulk1 D8h5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1146 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ulk1 d8h5/product/Cell Signaling Technology Inc
    Average 98 stars, based on 1146 article reviews
    anti ulk1 d8h5 - by Bioz Stars, 2026-03
    98/100 stars

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    ( A – E ) ATG5 ( A ), <t>ULK1</t> ( B ), ATG9a ( C ), P62 ( D ), or Beclin1 ( E ) was knocked down in HeLa cells with siRNA. followed by co-transfection with STING and CMV-L1-neo RT DNA. ORF1p was detected with Western blots at 48 h post transfection. A colony assay was performed to measure LINE-1 activity. The results of three independent experiments are presented in the bar graphs (mean ± SEM; paired t -test). Compared with 0 ng STING-Flag transfection, significant differences were observed at 50 ng ( p < 0.001) and 100 ng ( p < 0.001), independent of ATG5, ULK1, ATG9a, P62, or Beclin1 knockdown. ns non-significant; * p < 0.05; ** p < 0.01; *** p < 0.001.
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    ( A – E ) ATG5 ( A ), <t>ULK1</t> ( B ), ATG9a ( C ), P62 ( D ), or Beclin1 ( E ) was knocked down in HeLa cells with siRNA. followed by co-transfection with STING and CMV-L1-neo RT DNA. ORF1p was detected with Western blots at 48 h post transfection. A colony assay was performed to measure LINE-1 activity. The results of three independent experiments are presented in the bar graphs (mean ± SEM; paired t -test). Compared with 0 ng STING-Flag transfection, significant differences were observed at 50 ng ( p < 0.001) and 100 ng ( p < 0.001), independent of ATG5, ULK1, ATG9a, P62, or Beclin1 knockdown. ns non-significant; * p < 0.05; ** p < 0.01; *** p < 0.001.
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    ( A – E ) ATG5 ( A ), <t>ULK1</t> ( B ), ATG9a ( C ), P62 ( D ), or Beclin1 ( E ) was knocked down in HeLa cells with siRNA. followed by co-transfection with STING and CMV-L1-neo RT DNA. ORF1p was detected with Western blots at 48 h post transfection. A colony assay was performed to measure LINE-1 activity. The results of three independent experiments are presented in the bar graphs (mean ± SEM; paired t -test). Compared with 0 ng STING-Flag transfection, significant differences were observed at 50 ng ( p < 0.001) and 100 ng ( p < 0.001), independent of ATG5, ULK1, ATG9a, P62, or Beclin1 knockdown. ns non-significant; * p < 0.05; ** p < 0.01; *** p < 0.001.
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    ( A – E ) ATG5 ( A ), ULK1 ( B ), ATG9a ( C ), P62 ( D ), or Beclin1 ( E ) was knocked down in HeLa cells with siRNA. followed by co-transfection with STING and CMV-L1-neo RT DNA. ORF1p was detected with Western blots at 48 h post transfection. A colony assay was performed to measure LINE-1 activity. The results of three independent experiments are presented in the bar graphs (mean ± SEM; paired t -test). Compared with 0 ng STING-Flag transfection, significant differences were observed at 50 ng ( p < 0.001) and 100 ng ( p < 0.001), independent of ATG5, ULK1, ATG9a, P62, or Beclin1 knockdown. ns non-significant; * p < 0.05; ** p < 0.01; *** p < 0.001.

    Journal: EMBO Reports

    Article Title: STING inhibits LINE-1 retrotransposition through sorting ORF1p to lysosomes for degradation

    doi: 10.1038/s44319-025-00551-0

    Figure Lengend Snippet: ( A – E ) ATG5 ( A ), ULK1 ( B ), ATG9a ( C ), P62 ( D ), or Beclin1 ( E ) was knocked down in HeLa cells with siRNA. followed by co-transfection with STING and CMV-L1-neo RT DNA. ORF1p was detected with Western blots at 48 h post transfection. A colony assay was performed to measure LINE-1 activity. The results of three independent experiments are presented in the bar graphs (mean ± SEM; paired t -test). Compared with 0 ng STING-Flag transfection, significant differences were observed at 50 ng ( p < 0.001) and 100 ng ( p < 0.001), independent of ATG5, ULK1, ATG9a, P62, or Beclin1 knockdown. ns non-significant; * p < 0.05; ** p < 0.01; *** p < 0.001.

    Article Snippet: Rabbit anti-ULK1 antibody , Proteintech , 20986-1-AP.

    Techniques: Cotransfection, Western Blot, Transfection, Colony Assay, Activity Assay, Knockdown